基本信息

姓名：徐成冉

职称：博雅计划特聘教授/研究员

院系：北京大学基础医学院；北大-清华生命科学联合中心；北京市海淀区学院路38号，100191

联系电话：010-82805312

电子邮箱：cxu@pku.edu.cn

教育经历

2009-2013，博士后，细胞和发育生物学，美国宾夕法尼亚大学

2008-2009，博士后，细胞和发育生物学，美国Fox Chase癌症中心

2005-2008，博士后，免疫学，美国Scripps研究所

2000-2005，博士，植物学，北京大学

1995-1999，学士，遗传学，复旦大学

工作经历

2024-至今，特聘研究员，内蒙古大学生物医学研究院

2023-至今，长聘教授/研究员，北京大学基础医学院

2021-2023，研究员，北京大学基础医学院

2013-2020，研究员，北京大学生命科学学院  

2013-至今，研究员，北大-清华生命科学联合中心

研究方向

主要从事哺乳动物内胚层细胞向肝实质细胞和胰腺细胞分化和成熟调控机制的研究。近年来系统的研究了胰腺前体细胞、内分泌前体细胞、胰岛细胞和肝母细胞体内精确的分化路径和遗传、表观遗传调控机制，鉴定了调控细胞分化的多个细胞信号通路。优化了胚胎干细胞向肝脏和胰岛细胞体外分化效率，用于肝病和糖尿病的细胞治疗。

著作（#共同一作，*通讯作者）

Xin-Xin Yu^#, Xin Wang^#, Wei-Lin Qiu^#, Liu Yang and Cheng-Ran Xu* (2024). Stem Cell Therapy for Diabetes. Chapter 2: Genetic regulatory networks guiding islet development (ed. L. Piemonti, J. Odorico, T. J. Kieffer, V. Sordi and E. de Koning), Springer Nature, Switzerland AG (2024)

研究论文（#共同一作，*通讯作者）

通讯作者：

1. Ke-Ran Li^#, Pei-Long Yu^#, Qi-Qi Zheng^#, Xin Wang, Xuan Fang, Lin-Chen Li and Cheng-Ran Xu^*, Spatiotemporal and genetic cell lineage tracing of endodermal organogenesis at single-cell resolution. Cell, 188(3):796-813 (2025)

2. Liu Yang^#, Xin-Xin Yu^#, Xin Wang, Chen-Tao Jin and Cheng-Ran Xu^*, The expression order determines the pioneer functions of Ngn3 and NeuroD1 in pancreatic endocrine differentiation. Science Advances (in press)

3. Yu Zhang^#, Shuang He^#, Xi Wang, Xin Wang, Mao-Yang He, Xin-Xin Yu and Cheng-Ran Xu^*, ACSS2 mediates an epigenetic pathway to regulate β-cell adaptation during gestation. Nature Communications (in press)

4. Li Yang^#, Xin Wang^#, Jia-Xi Zheng, Zi-Ran Xu, Lin-Chen Li, Yu-Long Xiong, Bi-Chen Zhou, Jie Gao and Cheng-Ran Xu^*, Determination of key events in hepatocyte maturation at the single-cell level. Developmental Cell, 58(19):1996-2010 (2023)

5. Li Yang^#, Xin Wang^#, Xin-Xin Yu, Lu Yang, Jing Yang and Cheng-Ran Xu^*, The default and directed pathways of bipotent hepatoblast differentiation involve distinct epigenomic mechanisms. Developmental Cell, 58(18): 1688-1700 (2023)

6. Xin-Xin Yu^#, Wei-Lin Qiu^#, Liu Yang^#, Yan-Chun Wang^#, Mao-Yang He, Dan Wang, Yu Zhang, Lin-Chen Li, Jing Zhang, and Yi Wang and Cheng-Ran Xu^*, Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans. Cell Research, 31(8): 886-903 (2021).

7. Lin-Chen Li^#, Xin Wang^#, Zi-Ran Xu^#, Yan-Chun Wang^#, Ye Feng, Liu Yang, Wei-Lin Qiu, Li Yang, Xin-Xin Yu, Jun Gu and Cheng-Ran Xu^*, Single-cell patterning and axis characterization in the murine and human definitive endoderm. Cell Research, 31(3): 326-344 (2021).

8. Sisi Feng^#, Jiaying Wu^#, Wei-Lin Qiu^#, Li Yang^#, Xiaogang Deng^#, Ying Zhou, Yabin Chen, Xiao Li, Lei Yu, Zi-Ran Xu, Yini Xiao, Xiongzhao Ren, Ludi Zhang, Chenhua Wang, Xiaoyan Ding, Yuelei Chen, Paul Gadue, Guoyu Pan, Mina Ogawa, Shinichiro Ogawa, Jie Na, Lijian Hui, Hao Yin^*, Luonan Chen^*, Cheng-Ran Xu^* and Xin Cheng^*, Large-scale generation of functional and transplantable hepatocytes and cholangiocytes from human endoderm stem cells. Cell Reports, 33(10):108455 (2020).

9. Xin Wang^#, Li Yang^#, Yan-Chun Wang^#, Zi-Ran Xu, Ye Feng, Jing Zhang, Yi Wang and Cheng-Ran Xu^*, Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Research, 30(12):1109-1126 (2020).

10. Xin-Xin Yu and Cheng-Ran Xu^*, Understanding generation and regeneration of pancreatic β cells from a single-cell perspective (Review). Development, 12;147(7): dev179051 (2020).

11. Ye Feng^#, Wei-Lin Qiu^#, Xin-Xin Yu, Yu Zhang, Mao-Yang He, Weiyi Zhang, Michael Franti, Junqing Ye^*, Joerg D. Hoeck^* and Cheng-Ran Xu^*, Characterizing pancreatic β-cell heterogeneity in the streptozotocin model by single-cell transcriptomic analysis. Molecular Metabolism, 37:100982 (2020).

12. Xin-Xin Yu^#, Wei-Lin Qiu^#, Liu Yang, Yu Zhang, Mao-Yang He, Lin-Chen Li and Cheng-Ran Xu^*, Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution. The EMBO Journal, 15;38(8):e100164 (2019).

13. Li Yang, Lamaqiezhong and Cheng-Ran Xu^*，Molecular regulation mechanism of hepatic lineage differentiation and maturation. Chinese Journal of Cell Biology, 41(10):1853-1884 (2019).

14. Li Yang, Lin-Chen Li, Lamaoqiezhong, Xin Wang, Wei-Hua Wang, Yan-Chun Wang and Cheng-Ran Xu^*, The contributions of mesoderm-derived cells in liver development. Seminars in Cell and Developmental Biology, 92:63-76 (2019).

15. Lin-Chen Li^#, Wei-Lin Qiu^#, Yu-Wei Zhang, Zi-Ran Xu, Yi-Ni Xiao, Caiying Hou, Lamaoqiezhong, Peng Yu, Xin Cheng and Cheng-Ran Xu^*, Single-cell transcriptomic analyses reveal distinct dorsal/ventral pancreatic programs. EMBO Reports, 19(10):e46148 (2018).

16. Lin-Chen Li^#, Xin-Xin Yu^#, Yu-Wei Zhang^#, Ye Feng^#, Wei-Lin Qiu^# and Cheng-Ran Xu^*, Single-cell transcriptomic analyses of mouse pancreatic endocrine cells. Journal of Visualized Experiments, Sep 30;(139):58000 (2018).

17. Xin-Xin Yu^#, Wei-Lin Qiu^#, Liu Yang, Lin-Chen Li, Yu-Wei Zhang and Cheng-Ran Xu^*, Dynamics of chromatin marks and the role of JMJD3 during pancreatic endocrine cell fate commitment. Development, 145(6):dev163162 (2018).

18. Li Yang^#, Wei-Hua Wang^#, Wei-Lin Qiu^#, Zhen Guo, Erfei Bi and Cheng-Ran Xu^*, A single-cell transcriptomic analysis reveals precise pathways and regulatory mechanisms underlying hepatoblast differentiation. Hepatology, 66(5):1387-1401 (2017).

19. Wei-Lin Qiu^#, Yu-Wei Zhang^#, Ye Feng^#, Lin-Chen Li, Liu Yang and Cheng-Ran Xu^*, Deciphering pancreatic islet β-cell and α-cell maturation pathways and characteristic features at the single-cell level. Cell Metabolism, 25:1194-1205 (2017).

第一作者/合作作者:

20. Haobin Jiang, Lina Bai, Shen Song, Qianqian Yin, Anteng Shi, Bin Zhou, Hong Lian, Houzao Chen, Cheng-Ran Xu, Yanchun Wang, Yu Nie, Shengshou Hu, EZH2 controls epicardial cell migration during heart development. Life Sci Alliance, 10;6(6) (2023).

21. Suwei Gao^#, Qiang Shi^#, Yifan Zhang, Guixian Liang, Zhixin Kang, Baofeng Huang, Dongyuan Ma, Lu Wang, Jianwei Jiao, Xiangdong Fang, Cheng-Ran Xu, Longqi Liu, Xun Xu, Berthold Göttgens, Cheng Li^*, Feng Liu^*, Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics. Cell Research, 32(1): 38-53 (2022).

22. Jaesung P. Choi, Xi Yang, Shuang He, Renhua Song, Zi-Ran Xu, Matthew Foley, Justin Wong, Cheng-Ran Xu and Xiangjian Zheng^*, CCM2L deletion aggravates cerebral cavernous malformation through MEKK3-KLF signalling pathway. Stroke, 52(4): 1428-1436 (2021).

23. Ramkumar Mohan, Yiping Mao, Shungang Zhang, Yuwei Zhang, Cheng-Ran Xu, Gérard Gradwohl and Xiaoqing Tang^*. Differentially expressed microRNA-483 confers distinct functions in pancreatic beta- and alpha-cells. Journal of Biological Chemistry, 290(32): 19955-66 (2015).

24. Cheng-Ran Xu, Lin-Chen Li, Greg Donahue, Lei Ying, Yu-Wei Zhang, Paul Gadue & Kenneth S. Zaret^*. Dynamics of Genomic H3K27me3 Domains and Role of EZH2 during Pancreatic Endocrine Specification. The EMBO Journal, 33(19): 2157-2170 (2014).

25. Cheng-Ran Xu & Kenneth S. Zaret^*. Chromatin "Pre-Pattern" and Epigenetic Modulation in the Cell Fate Choice of Liver over Pancreas in the Endoderm. Review, Nucleus, 3(2): 150-154 (2012).

26. Cheng-Ran Xu, Philip A. Cole, Jay D. Kormish, Shannon Dent & Kenneth S. Zaret^*. Chromatin "Pre-Pattern" and Histone Modifiers in a Fate Choice for Liver and Pancreas. Science, 332: 963-6 (2011).

27. Cheng-Ran Xu & Ann J. Feeney^*. The epigenetic profile of Ig genes is dynamically regulated during B cell differentiation and is modulated by pre-B cell receptor signaling. Journal of Immunology, 182(3): 1362-9 (2009).

28. Cheng-Ran Xu, Lana Schaffer, Steven R. Head & Ann J. Feeney^*. Reciprocal patterns of methylation of H3K27 and H3K36 on proximal vs. distal IgV_H genes are modulated by IL7 and Pax5. The Proceedings of the National Academy of Sciences USA (PNAS), 105: 8685-8690 (2008).

29. Cheng-Ran Xu, Cui Liu, Yi-Lan Wang, Lin-Chen Li, Wen-Qian Chen, Zhi-Hong Xu & Shu-Nong Bai^*. Histone acetylation affects expression of cellular patterning genes in the Arabidopsis root epidermis. The Proceedings of the National Academy of Sciences USA (PNAS), 102: 14469-14474 (2005).